Direct methods for studying transcription regulatory proteins and RNA polymerase in bacteria
Grainger, David C., Lee, David J. and Busby, Stephen J. W. (2009) Direct methods for studying transcription regulatory proteins and RNA polymerase in bacteria. Current Opinion in Microbiology, Vol.12 (No.5). pp. 531-535. ISSN 1369-5274Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.mib.2009.08.006
Transcription factors and sigma factors play a major role in bacterial gene regulation by guiding the distribution of RNA polymerase between the promoters of different transcription units in response to changes in the environment. For 40 years Escherichia coli K-12 has been the paradigm for investigating this regulation and most studies have focused on small numbers of promoters studied by a combination of genetics and biochemistry. Since the first complete sequence for a bacterial genome was reported, the emphasis has switched to studying transcription on a global scale, with transcriptomics and bioinformatics becoming the methods of choice. Here we discuss two complementary direct experimental methods for studying transcription factors and sigma factors and we outline their potential use in rapidly establishing the regulatory networks in newly sequenced bacteria.
|Item Type:||Journal Item|
|Subjects:||Q Science > QR Microbiology|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Current Opinion in Microbiology|
|Number of Pages:||5|
|Page Range:||pp. 531-535|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Wellcome Trust, Wellcome Trust Research Career Development Fellowship|
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