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Evolutionarily conserved regulatory motifs in the promoter of the Arabidopsis clock gene LATE ELONGATED HYPOCOTYL
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Spensley, Mark, Kim, Jae-Yean, Picot, Emma, Reid, John, Ott, Sascha, Helliwell, Chris and Carré, Isabelle A.. (2009) Evolutionarily conserved regulatory motifs in the promoter of the Arabidopsis clock gene LATE ELONGATED HYPOCOTYL. Plant Cell, Vol.21 (No.9). pp. 2606-2623. ISSN 1040-4651
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Official URL: http://dx.doi.org/10.1105/tpc.109.069898
Abstract
The transcriptional regulation of the LATE ELONGATED HYPOCOTYL (LHY) gene is key to the structure of the circadian oscillator, integrating information from multiple regulatory pathways. We identified a minimal region of the LHY promoter that was sufficient for rhythmic expression. Another upstream sequence was also required for appropriate waveform of transcription and for maximum amplitude of oscillations under both diurnal and free-running conditions. We showed that two classes of protein complexes interact with a G-box and with novel 5A motifs; mutation of these sites reduced the amplitude of oscillation and broadened the peak of expression. A genome-wide bioinformatic analysis showed that these sites were enriched in phase-specific clusters of rhythmically expressed genes. Comparative genomic analyses showed that these motifs were conserved in orthologous promoters from several species. A position-specific scoring matrix for the 5A sites suggested similarity to CArG boxes, which are recognized by MADS box transcription factors. In support of this, the FLOWERING LOCUS C (FLC) protein was shown to interact with the LHY promoter in planta. This suggests a mechanism by which FLC might affect circadian period.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry S Agriculture > SB Plant culture Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Centre for Systems Biology |
| Journal or Publication Title: | Plant Cell |
| Publisher: | American Society of Plant Biologists |
| ISSN: | 1040-4651 |
| Date: | September 2009 |
| Volume: | Vol.21 |
| Number: | No.9 |
| Number of Pages: | 18 |
| Page Range: | pp. 2606-2623 |
| Identification Number: | 10.1105/tpc.109.069898 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Research Councils UK (RCUK), Gatsby Foundation |
| Grant number: | 88/G07884 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/17039 |
Data sourced from Thomson Reuters' Web of Knowledge
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