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Insight into early events in the aggregation of the prion protein on lipid membranes
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Sanghera, Narinder, Swann, Marcus J., Ronan, Gerry and Pinheiro, Teresa J. T. (2009) Insight into early events in the aggregation of the prion protein on lipid membranes. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1788 (No.10). pp. 2245-2251. doi:10.1016/j.bbamem.2009.08.005
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Official URL: http://dx.doi.org/10.1016/j.bbamem.2009.08.005
Abstract
The key molecular event underlying prion diseases is the conversion of the monomeric and alpha-helical cellular form of the prion protein (PrPC) to the disease-associated state, which is aggregated and rich in beta-sheet (PrPSc). The molecular details associated with the conversion of PrPC into PrPSc are not fully understood. The prion protein is attached to the cell membrane via a GPI lipid anchor and evidence suggests that the lipid environment plays an important role in prion conversion and propagation. We have previously shown that the interaction of the prion protein with anionic lipid membranes induces beta-sheet structure and promotes prion aggregation, whereas zwitterionic membranes stabilize the alpha-helical form of the protein. Here, we report on the interaction of recombinant sheep prion protein with planar lipid membranes in real-time, using dual polarization interferometry (DPI). Using this technique, the simultaneous evaluation of multiple physical properties of PrP layers on membranes was achieved. The deposition of prion on membranes of POPC and POPC/POPS mixtures was studied. The properties of the resulting protein layers were found to depend on the lipid composition of the membranes. Denser and thicker protein deposits formed on lipid membranes containing POPS compared to those formed on POPC. DPI thus provides a further insight on the organization of PrP at the surface of lipid membranes. (C) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Biomembranes | ||||
| Publisher: | Elsevier BV | ||||
| ISSN: | 00052736 | ||||
| Official Date: | October 2009 | ||||
| Dates: |
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| Volume: | Vol.1788 | ||||
| Number: | No.10 | ||||
| Number of Pages: | 7 | ||||
| Page Range: | pp. 2245-2251 | ||||
| DOI: | 10.1016/j.bbamem.2009.08.005 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
| Grant number: | IIP0206/008 |
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