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Interactions in vivo between the Vif protein of HIV-1 and the precursor (Pr55(GAG)) of the virion nucleocapsid proteins
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Syed, Farhatullah and McCrae, Malcolm A.. (2009) Interactions in vivo between the Vif protein of HIV-1 and the precursor (Pr55(GAG)) of the virion nucleocapsid proteins. Archives of Virology, Vol.154 (No.11). pp. 1797-1805. ISSN 0304-8608
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Official URL: http://dx.doi.org/10.1007/s00705-009-0520-8
Abstract
The abnormality of viral core structure seen in vif-defective HIV-1 grown in PBMCs has suggested a role for Vif in viral morphogenesis. Using an in vivo mammalian two-hybrid assay, the interaction between Vif and the precursor (Pr55(GAG)) of the virion nucleocapsid proteins has been analysed. This revealed the amino-terminal (aa 1-22) and central (aa 70-100) regions of Vif to be essential for its interaction with Pr55(GAG), but deletion of the carboxy-terminal (aa 158-192) region of the protein had only a minor effect on its interaction. Initial deletion studies carried out on Pr55(GAG) showed that a 35-amino-acid region of the protein bridging the MA(p17)-CA(p24) junction was essential for its ability to interact with Vif. Site-directed mutagenesis of a conserved tryptophan (Trp(21)) near the amino terminus of Vif showed it to be important for the interaction with Pr55(GAG). By contrast, mutagenesis of the highly conserved YLAL residues forming part of the BC-box motif, shown to be important in Vif promoting degradation of APOBEC3G/3F, had little or no effect on the Vif-Pr55(GAG) interaction.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) |
| Journal or Publication Title: | Archives of Virology |
| Publisher: | Springer Wien |
| ISSN: | 0304-8608 |
| Date: | November 2009 |
| Volume: | Vol.154 |
| Number: | No.11 |
| Number of Pages: | 9 |
| Page Range: | pp. 1797-1805 |
| Identification Number: | 10.1007/s00705-009-0520-8 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/17105 |
Data sourced from Thomson Reuters' Web of Knowledge
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