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Methodology for measuring conformation of solvent-disrupted protein subunits using T-WAVE ion mobility MS: an investigation into eukaryotic initiation factors

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Leary, Julie A., Schenauer, Matthew R., Stefanescu, Raluca, Andaya, Armann, Ruotolo, Brandon T., Robinson, Carol V., Thalassinos, Konstantinos, Scrivens, James H., Sokabe, Masaaki and Hershey, John W. B. (2009) Methodology for measuring conformation of solvent-disrupted protein subunits using T-WAVE ion mobility MS: an investigation into eukaryotic initiation factors. Journal of The American Society for Mass Spectrometry, Vol.20 (No.9). pp. 1699-1706. doi:10.1016/j.jasms.2009.05.003

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Official URL: http://dx.doi.org/10.1016/j.jasms.2009.05.003

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Abstract

The methodology developed in the research presented herein makes use of chaotropic solvents to gently dissociate subunits from an intact macromolecular complex and subsequently allows for the measurement of collision cross section (CCS) for both the recombinant (R-eIF3k) and solvent dissociated form of the subunit (S-eIF3k). In this particular case, the k Subunit from the eukaryotic initiation factor 3 (eIF3) was investigated in detail. Experimental and theoretical CCS values show both the recombinant and solvent disrupted forms of the protein to be essentially the same. The ultimate goal of the project is to structurally characterize all the binding partners of eIF3, determine which subunits interact directly, and investigate how subunits may change conformation when they form complexes with other proteins. Research presented herein is the first report showing retention of solution conformation of a protein as evidenced by CCS measurements of both recombinant and solvent disrupted versions of the same protein. (J Am Soc Mass Spectrom 2009, 20, 1699-1706) (C) 2009 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QC Physics
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title: Journal of The American Society for Mass Spectrometry
Publisher: Springer New York LLC
ISSN: 1044-0305
Official Date: September 2009
Dates:
DateEvent
September 2009UNSPECIFIED
Volume: Vol.20
Number: No.9
Number of Pages: 8
Page Range: pp. 1699-1706
DOI: 10.1016/j.jasms.2009.05.003
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: NIH PHS, Human Frontier Science Program Fellowship
Grant number: GM073732, LT00575/2007

Data sourced from Thomson Reuters' Web of Knowledge

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