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Hot electron capture dissociation distinguishes leucine from lsoleucine in a novel hemoglobin variant, Hb Askew, beta 54(D5)Val -> Ile
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Williams, Jonathan P., Creese, Andrew J., Roper, David R., Green, Brian N. and Cooper, Helen J. (2009) Hot electron capture dissociation distinguishes leucine from lsoleucine in a novel hemoglobin variant, Hb Askew, beta 54(D5)Val -> Ile. Journal of The American Society for Mass Spectrometry, Vol.20 (No.9). pp. 1707-1713. doi:10.1016/j.jasms.2009.05.002 ISSN 1044-0305.
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Official URL: http://dx.doi.org/10.1016/j.jasms.2009.05.002
Abstract
Population migration has led to the global dispersion of human hemoglobinopathies and has precipitated a need for their identification. An effective mass spectrometry-based procedure involves analysis of the intact alpha- and beta-globin chains to determine their mass, followed by location of the variant amino acid residue by direct analysis of the enzymatically digested chains and low-energy collision induced dissociation of the variant peptide. Using this procedure, a variant was identified as either beta 54Val -> Leu or beta 54Val -> Ile, since the amino acids leucine and isoleucine cannot be distinguished using low-energy collisions. Here, we describe how hot electron capture dissociation on a Fourier transform-ion cyclotron resonance mass spectrometer was used to distinguish isoleucine from leucine and identify the mutation as beta 54(D5)Val -> Ile. This is a novel variant, and we have named it Hb Askew. (J Am Soc Mass Spectrom 2009, 20, 1707-1713) (C) 2009 American Society for Mass Spectrometry
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QC Physics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
Publisher: | Springer New York LLC | ||||
ISSN: | 1044-0305 | ||||
Official Date: | September 2009 | ||||
Dates: |
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Volume: | Vol.20 | ||||
Number: | No.9 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 1707-1713 | ||||
DOI: | 10.1016/j.jasms.2009.05.002 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Warwick University Research Development Fund, Wellcome Trust | ||||
Grant number: | 074131 |
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