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Functional applications of stable Tau Oligomers in cell biology and electrophysiology studies
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Hill, Emily, Moffat, Kevin G., Wall, Mark J., Zetterberg, Henrik, Blennow, Kaj and Karikari, Thomas K. (2023) Functional applications of stable Tau Oligomers in cell biology and electrophysiology studies. In: Protein Aggregation. Methods in Molecular Biology, 2551 . New York, NY: Humana, pp. 147-161. ISBN 9781071625972
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Official URL: https://doi.org/10.1007/978-1-0716-2597-2_11
Abstract
Aggregated tau protein plays a key role in the pathogenesis of neurodegenerative tauopathies including Alzheimer's disease. Soluble, low-molecular-weight tau oligomers are formed early in disease processes and are thought to have toxic functions that disrupt neuronal function. The dynamic and transient nature of tau oligomers complicates in vitro functional studies to explore the mechanistic links between oligomer formation and neurodegeneration. We have previously described a method of producing stable and structurally characterized oligomers that maintain their oligomeric conformation and prevent further aggregation. This method allows for the flexibility of stabilizing tau oligomers by specifically labelling cysteine residues with fluorescent or colorless maleimide conjugates. Here, we describe the functional applications of these preformed stable tau oligomers in cell biology and electrophysiological studies. These investigations allow real-time insights into the cellular uptake of exogenous tau oligomers and their functional effects in the recipient cells. [Abstract copyright: © 2023. Springer Science+Business Media, LLC, part of Springer Nature.]
| Item Type: | Book Item | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| SWORD Depositor: | Library Publications Router | ||||
| Series Name: | Methods in Molecular Biology | ||||
| Journal or Publication Title: | Methods in Molecular Biology | ||||
| Publisher: | Humana | ||||
| Place of Publication: | New York, NY | ||||
| ISBN: | 9781071625972 | ||||
| ISSN: | 1064-3745 | ||||
| Book Title: | Protein Aggregation | ||||
| Official Date: | 31 October 2023 | ||||
| Dates: |
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| Volume: | 2551 | ||||
| Page Range: | pp. 147-161 | ||||
| DOI: | 10.1007/978-1-0716-2597-2_11 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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