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Nucleic acid aptamers as aptasensors for plant biology
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Tungsirisurp, Sireethorn, O’Reilly, Rachel K. and Napier, Richard (2023) Nucleic acid aptamers as aptasensors for plant biology. Trends in Plant Science, 28 (3). pp. 359-371. doi:10.1016/j.tplants.2022.10.002 ISSN 1360-1385.
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WRAP-Nucleic-acid-aptamers-aptasensors-plant-biology-22.pdf - Publisher's Proof Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1728Kb) | Preview |
Official URL: https://doi.org/10.1016/j.tplants.2022.10.002
Abstract
Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QK Botany Q Science > QP Physiology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Library of Congress Subject Headings (LCSH): | Nucleic acids, Nucleotide sequence , Biosensors , Plant hormones -- Synthesis | ||||||||
| Journal or Publication Title: | Trends in Plant Science | ||||||||
| Publisher: | Elsevier BV | ||||||||
| ISSN: | 1360-1385 | ||||||||
| Official Date: | March 2023 | ||||||||
| Dates: |
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| Volume: | 28 | ||||||||
| Number: | 3 | ||||||||
| Page Range: | pp. 359-371 | ||||||||
| DOI: | 10.1016/j.tplants.2022.10.002 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 19 December 2022 | ||||||||
| Date of first compliant Open Access: | 19 December 2022 | ||||||||
| RIOXX Funder/Project Grant: |
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