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Imaging cancer cells using a holographic microscope
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Zhao, Yikun and Bryansyon-Cross, Peter (2022) Imaging cancer cells using a holographic microscope. In: IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Tianjin, China, 08-12 Aug 2022. Published in: 2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) pp. 385-390. ISBN 9781665475440. doi:10.1109/3m-nano56083.2022.9941675 ISSN 2373-5422.
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Official URL: https://doi.org/10.1109/3m-nano56083.2022.9941675
Abstract
In this paper, a compact and high resolution in-line digital holographic microscope (DHM) is constructed that consisted of a 532 nm laser, mirrors, microscope objectives (10×,NA=0.25 and 40×,NA=0.65 ) and CCD camera. Using the system, a series of holograms were successfully obtained and nμmerically reconstructed to extract the three-dimensional measurements of phase, amplitude and the morphological structure of the tested cells. In this method, the quantitative biological measurements of different types cured cancer cells, involving A549, MCF-7 and SMMC-7721 cells, were all estimated to reveal biological structural information. Moreover, quantitative analysis of SMMC-7721 cell growth changes and drug response were discussed to supply significant information for the treatment of cancer cells.
Item Type: | Conference Item (Paper) | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Journal or Publication Title: | 2022 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) | ||||||
Publisher: | IEEE | ||||||
ISBN: | 9781665475440 | ||||||
ISSN: | 2373-5422 | ||||||
Official Date: | 16 November 2022 | ||||||
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Page Range: | pp. 385-390 | ||||||
DOI: | 10.1109/3m-nano56083.2022.9941675 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Copyright Holders: | IEEE | ||||||
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Conference Paper Type: | Paper | ||||||
Title of Event: | IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) | ||||||
Type of Event: | Conference | ||||||
Location of Event: | Tianjin, China | ||||||
Date(s) of Event: | 08-12 Aug 2022 | ||||||
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