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Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin
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Alruwaili, Awatif, Rashid, Goran M. M., Sodré, Victoria, Mason, James, Rehman, Zainab, Menakath, Anjali K., Cheung, David L., Brown, Steven P. and Bugg, Timothy D. H. (2023) Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin. RSC Chemical Biology, 4 (1). pp. 47-55. doi:10.1039/d2cb00173j ISSN 2633-0679.
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WRAP-Elucidation-of-microbial-lignin-degradation-pathways-using-synthetic-isotope-labelled-lignin-Bugg-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1191Kb) | Preview |
Official URL: https://doi.org/10.1039/D2CB00173J
Abstract
Pathways by which the biopolymer lignin is broken down by soil microbes could be used to engineer new biocatalytic routes from lignin to renewable chemicals, but are currently not fully understood. In order to probe these pathways, we have prepared synthetic lignins containing 13C at the sidechain β-carbon. Feeding of [β-13C]-labelled DHP lignin to Rhodococcus jostii RHA1 has led to the incorporation of 13C label into metabolites oxalic acid, 4-hydroxyphenylacetic acid, and 4-hydroxy-3-methoxyphenylacetic acid, confirming that they are derived from lignin breakdown. We have identified a glycolate oxidase enzyme in Rhodococcus jostii RHA1 which is able to oxidise glycolaldehyde via glycolic acid to oxalic acid, thereby identifying a pathway for the formation of oxalic acid. R. jostii glycolate oxidase also catalyses the conversion of 4-hydroxyphenylacetic acid to 4-hydroxybenzoylformic acid, identifying another possible pathway to 4-hydroxybenzoylformic acid. Formation of labelled oxalic acid was also observed from [β-13C]-polyferulic acid, which provides experimental evidence in favour of a radical mechanism for α,β-bond cleavage of β-aryl ether units.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QK Botany T Technology > TP Chemical technology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Physics |
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| SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Biopolymers, Lignin -- Biodegradation, Biodegradation | ||||||||||||||||||||||||
| Journal or Publication Title: | RSC Chemical Biology | ||||||||||||||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||||||||||||||
| ISSN: | 2633-0679 | ||||||||||||||||||||||||
| Official Date: | 1 January 2023 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 4 | ||||||||||||||||||||||||
| Number: | 1 | ||||||||||||||||||||||||
| Page Range: | pp. 47-55 | ||||||||||||||||||||||||
| DOI: | 10.1039/d2cb00173j | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
| Date of first compliant deposit: | 5 December 2022 | ||||||||||||||||||||||||
| Date of first compliant Open Access: | 5 December 2022 | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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