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Flow linear dichroism of some prototypical proteins
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Bulheller, Benjamin M., Rodger, Alison, Hicks, Matthew R., Dafforn, Tim, Serpell, Louise C., Marshall, Karen E., Bromley, Elizabeth H. C., King, Patrick J. S., Channon, Kevin J., Woolfson, Derek N. and Hirst, Jonathan D. (2009) Flow linear dichroism of some prototypical proteins. Journal of the American Chemical Society, Vol.131 (No.37). pp. 13305-13314. doi:10.1021/ja902662e ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja902662e
Abstract
Flow linear dichroism (LD) spectroscopy provides information on the orientation of molecules in solution and hence on the relative orientation of parts of molecules. Long molecules such as fibrous proteins can be aligned in Couette flow cells and characterized using LD. We have measured using Couette flow and calculated from first principles the LD of proteins representing prototypical secondary structure classes: a self-assembling fiber,and tropomyosin (all-alpha-helical), FtsZ (an alpha beta protein), an amyloid fibril (beta-sheet), and Collagen [poly(proline)II helices]. the combination of calculation and experiment allows elucidation of the protein orientation in the Couette flow and the orientation of chromophores within the protein fibers.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Linear dichroism, Proteins -- Spectra | ||||
Journal or Publication Title: | Journal of the American Chemical Society | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 0002-7863 | ||||
Official Date: | 23 September 2009 | ||||
Dates: |
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Volume: | Vol.131 | ||||
Number: | No.37 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 13305-13314 | ||||
DOI: | 10.1021/ja902662e | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC) | ||||
Grant number: | GR/T09224 (EPSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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