The Library
Discrimination between protein glycoforms using lectin-functionalised gold nanoparticles as signal enhancers
Tools
Neves, Marta, Richards, Sarah-Jane, Baker, Alexander N., Walker, Marc, Georgiou, Panagiotis G. and Gibson, Matthew I. (2023) Discrimination between protein glycoforms using lectin-functionalised gold nanoparticles as signal enhancers. Nanoscale Horizons, 8 (3). pp. 377-382. doi:10.1039/D2NH00470D ISSN 2055-6756.
|
PDF
d2nh00470d.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1291Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/D2NH00470D
Abstract
Glycoforms (and other post-translational modifications) of otherwise identical proteins can indicate pathogenesis/disease state and hence new tools to detect and sense a protein's glycosylation status are essential. Antibody-based assays against specific protein sequences do not typically discriminate between glycoforms. Here we demonstrate a ‘sandwich’ bio-assay approach, whereby antibodies immobilised onto biolayer interferometry sensors first select proteins, and then the specific glycoform is identified using gold nanoparticles functionalised with lectins which provide signal enhancement. The nanoparticles significantly enhance the signal relative to lectins alone, allowing glycoform specific detection as low as 0.04 μg mL−1 (1.4 nM) in buffer, and crucially there is no need for an enrichment step and all steps can be automated. Proof of concept is demonstrated using prostate specific antigen: a biomarker for prostate cancer, where glycoform analysis could distinguish between cancerous and non-cancerous status, rather than only detecting overall protein concentration.
Item Type: | Journal Article | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||
Library of Congress Subject Headings (LCSH): | Glycoproteins, Glycoproteins -- Analysis, Proteomics , Proteins -- Analysis, Lectins -- Therapeutic use | ||||||||||||
Journal or Publication Title: | Nanoscale Horizons | ||||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||||
ISSN: | 2055-6756 | ||||||||||||
Official Date: | 1 March 2023 | ||||||||||||
Dates: |
|
||||||||||||
Volume: | 8 | ||||||||||||
Number: | 3 | ||||||||||||
Page Range: | pp. 377-382 | ||||||||||||
DOI: | 10.1039/D2NH00470D | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 19 January 2023 | ||||||||||||
Date of first compliant Open Access: | 19 January 2023 | ||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year