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Motor generated torque drives coupled yawing and orbital rotations of kinesin coated gold nanorods
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Sugawa, Mitsuhiro, Maruyama, Yohei, Yamagishi, Masahiko, Cross, Robert A. and Yajima, Junichiro (2022) Motor generated torque drives coupled yawing and orbital rotations of kinesin coated gold nanorods. Communications Biology, 5 (1). 1368. doi:10.1038/s42003-022-04304-w ISSN 2399-3642.
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WRAP-motor-generated-torque-drives-coupled-yawing-orbital-rotations-kinesin-coated-gold-nanorods-Cross-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2013Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/s42003-022-04304-w
Abstract
Kinesin motor domains generate impulses of force and movement that have both translational and rotational (torque) components. Here, we ask how the torque component influences function in cargo-attached teams of weakly processive kinesins. Using an assay in which kinesin-coated gold nanorods (kinesin-GNRs) translocate on suspended microtubules, we show that for both single-headed KIF1A and dimeric ZEN-4, the intensities of polarized light scattered by the kinesin-GNRs in two orthogonal directions periodically oscillate as the GNRs crawl towards microtubule plus ends, indicating that translocating kinesin-GNRs unidirectionally rotate about their short (yaw) axes whilst following an overall left-handed helical orbit around the microtubule axis. For orientations of the GNR that generate a signal, the period of this short axis rotation corresponds to two periods of the overall helical trajectory. Torque force thus drives both rolling and yawing of near-spherical cargoes carrying rigidly-attached weakly processive kinesins, with possible relevance to intracellular transport.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QP Physiology | |||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Kinesin, Nanotechnology | |||||||||||||||||||||
Journal or Publication Title: | Communications Biology | |||||||||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||||||||
ISSN: | 2399-3642 | |||||||||||||||||||||
Official Date: | 20 December 2022 | |||||||||||||||||||||
Dates: |
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Volume: | 5 | |||||||||||||||||||||
Number: | 1 | |||||||||||||||||||||
Article Number: | 1368 | |||||||||||||||||||||
DOI: | 10.1038/s42003-022-04304-w | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 1 February 2023 | |||||||||||||||||||||
Date of first compliant Open Access: | 2 February 2023 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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