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Insulin and metformin regulate circulating and adipose tissue chemerin
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Tan, Bee K., Chen, Jing, Farhatullah, S., Adya, Raghu, Kaur, Jaspreet, Heutling, Dennis, Lewandowski, Krzysztof C., O'Hare, J. Paul, Lehnert, Hendrik and Randeva, Harpal S. (2009) Insulin and metformin regulate circulating and adipose tissue chemerin. Diabetes, Vol.58 (No.9). pp. 1971-1977. doi:10.2337/db08-1528 ISSN 0012-1797.
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Official URL: http://dx.doi.org/10.2337/db08-1528
Abstract
OBJECTIVE-To assess chemerin levels and regulation in sera and adipose tissue from women with polycystic ovary syndrome (PCOS) and matched control subjects.
RESEARCH DESIGN AND METHODS-Real-time RT-PCR and Western blotting were used to assess mRNA and protein expression of chemerin. Serum chemerin was measured by enzyme-linked immunosorbent assay. We investigated the in vivo effects of insulin on serum chemerin levels via a prolonged insulin-glucose infusion. Ex vivo effects of insulin, metformin, and steroid hormones on adipose tissue chemerin protein production and secretion into conditioned media were assessed by Western blotting and enzyme-linked immunosorbent assay, respectively.
RESULTS-Serum chemerin, subcutaneous, and omental adipose tissue chemerin were significantly higher in women with PCOS (n = 14; P < 0.05, P < 0.01). Hyperinsulinemic induction in human subjects significantly increased serum chemerin levels (n = 6; P < 0.05, P < 0.01). In adipose tissue explants, insulin significantly increased (n = 6; P < 0.05, P < 0.01) whereas metformin significantly decreased (n = 6; P < 0.05, P < 0.01) chemerin protein production and secretion into conditioned media, respectively. After 6 months of metformin treatment, there was a significant decrease in serum chemerin (n = 21; P < 0.01). Importantly, changes in homeostasis model assessment-insulin resistance were predictive of changes in serum chemerin (P = 0.046).
CONCLUSIONS-Serum and adipose tissue chemerin levels are increased in women with PCOS and are upregulated by insulin. Metformin treatment decreases serum chemerin in these women. Diabetes 58:1971-1977, 2009
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > RC Internal medicine | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Diabetes | ||||
| Publisher: | American Diabetes Association | ||||
| ISSN: | 0012-1797 | ||||
| Official Date: | September 2009 | ||||
| Dates: |
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| Volume: | Vol.58 | ||||
| Number: | No.9 | ||||
| Number of Pages: | 7 | ||||
| Page Range: | pp. 1971-1977 | ||||
| DOI: | 10.2337/db08-1528 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | General Charities of the City of Coventry |
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