Dulla, Chris G., Frenguelli, Bruno G., Staley, Kevin and Masino, Susan A. (2009) Intracellular acidification causes adenosine release during states of hyperexcitability in the hippocampus. Journal of Neurophysiology, Vol.102 (No.3). pp. 1984-1993. doi:10.1152/jn.90695.2008 ISSN 0022-3077.

Research output not available from this repository.

Request-a-Copy directly from author or use local Library Get it For Me service.

Request Changes to record.

Abstract

Dulla CG, Frenguelli BG, Staley KJ, Masino SA. Intracellular acidification causes adenosine release during states of hyperexcitability in the hippocampus. J Neurophysiol 102: 1984-1993, 2009. First published July 22, 2009; doi:10.1152/jn.90695.2008. Decreased pH increases extracellular adenosine in CNS regions as diverse as hippocampus and ventral medulla. However, thus far there is no clear consensus whether the critical pH change is a decrease in intracellular and/or extracellular pH. Previously we showed that a decrease in extracellular pH is necessary and a decrease in intracellular pH alone is not sufficient, to increase extracellular adenosine in an acute hippocampal slice preparation. Here we explored further the role of intracellular pH under different synaptic conditions in the hippocampal slice. When synaptic excitability was increased, either during gamma-aminobutyric acid type A receptor blockade in CA1 or after the induction of persistent bursting in CA3, a decrease in intracellular pH alone was now sufficient to: 1) elevate extracellular adenosine concentration, 2) activate adenosine A 1 receptors, 3) decrease excitatory synaptic transmission (CA1), and 4) attenuate burst frequency in an in vitro seizure model (CA3). Hippocampal slices obtained from adenosine A 1 receptor knockout mice did not exhibit these pH-mediated effects on synaptic transmission, further confirming the role of adenosine acting at the adenosine A 1 receptor. Taken together, these data strengthen and add significantly to the evidence outlining a change in pH as an important stimulus influencing extracellular adenosine. In addition, we identify conditions under which intracellular pH plays a dominant role in regulating extracellular adenosine concentrations.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Q Science > QP Physiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Adenosine, Hippocampus (Brain), Molecular neurobiology, Excitation (Physiology), Neural transmission
Journal or Publication Title:	Journal of Neurophysiology
Publisher:	American Physiological Society
ISSN:	0022-3077
Official Date:	September 2009
Dates:	Date Event September 2009 Published
Volume:	Vol.102
Number:	No.3
Number of Pages:	10
Page Range:	pp. 1984-1993
DOI:	10.1152/jn.90695.2008
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	American Epilepsy Foundation, Epilepsy Research UK, National Institute of Neurological Disorders and Stroke (U.S.), National Science Foundation (U.S.) (NSF), Howard Hughes Medical Institute
Grant number:	NS-34360-12 (NINDS), NS-29173 (NSF), NS-61290 (NSF)
Version or Related Resource:	Dulla, C.G., et al. (2008). Intracellular acidification causes adenosine release during states of hyperexcitability in the hippocampus. [Abstract]. Purinergic Signalling, 4(1), pp. S160-S161. http://wrap.warwick.ac.uk/id/eprint/29355
Related URLs:	Related item in WRAP

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item