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Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei
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Billington, Karen, Halliday, Clare, Madden, Ross, Dyer, Philip, Barker, Amy, Moreira-Leite, Flavia, Carrington, Mark, Vaughan, Sue, Hertz-Fowler, Christiane, Dean, Samuel, Sunter, Jack, Wheeler, Richard and Gull, Keith (2023) Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei. Nature microbiology, 8 . pp. 533-547. doi:10.1038/s41564-022-01295-6 ISSN 2058-5276.
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Official URL: https://doi.org/10.1038/s41564-022-01295-6
Abstract
Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and plant unicellular parasites. Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. We provide clues to function and define lineage-specific organelle adaptations for parasitism, mapping the ultraconserved cellular architecture of eukaryotes, including the first comprehensive ‘cartographic’ analysis of the eukaryotic flagellum, which is vital for morphogenesis and pathology. To demonstrate the power of this resource, we identify novel organelle subdomains and changes in molecular composition through the cell cycle. TrypTag is a transformative resource, important for hypothesis generation for both eukaryotic evolutionary molecular cell biology and fundamental parasite cell biology.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QL Zoology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Trypanosoma brucei , Eukaryotic cells -- Genetics, Cytology | |||||||||||||||||||||
| Journal or Publication Title: | Nature microbiology | |||||||||||||||||||||
| Publisher: | Nature Publishing Group | |||||||||||||||||||||
| ISSN: | 2058-5276 | |||||||||||||||||||||
| Official Date: | March 2023 | |||||||||||||||||||||
| Dates: |
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| Volume: | 8 | |||||||||||||||||||||
| Page Range: | pp. 533-547 | |||||||||||||||||||||
| DOI: | 10.1038/s41564-022-01295-6 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 27 February 2023 | |||||||||||||||||||||
| Date of first compliant Open Access: | 27 February 2023 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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