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Assessing amino acid uptake by phototrophic nanoflagellates in nonaxenic cultures using flow cytometric sorting
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Hartmann, Manuela, Zubkov, Mikhail V., Martin, Adrian P., Scanlan, David J. and Burkill, Peter H. (2009) Assessing amino acid uptake by phototrophic nanoflagellates in nonaxenic cultures using flow cytometric sorting. FEMS Microbiology Letters, Vol.298 (No.2). pp. 166-173. doi:10.1111/j.1574-6968.2009.01715.x ISSN 0378-1097.
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Official URL: http://dx.doi.org/10.1111/j.1574-6968.2009.01715.x
Abstract
Biologically available concentrations of individual dissolved amino acids in the open ocean are generally < 1 nM. Despite this, the microbial turnover of amino acids is usually measured in hours indicating high demand. It is thought that the majority of uptake is due to bacterioplankton, although protists, particularly phototrophic protists, are also expected to take up amino acids. In order to assess the ability of protists to compete with prokaryotes for amino acids at subnanomolar concentrations, we examined the direct uptake of H-3-leucine by phototrophic nanoflagellates (prasinophytes, pelagophytes and trebouxiophytes) and by associated bacteria using flow cytometric cell sorting. In contrast to H-3-leucine-assimilating bacterial copopulations, none of the six studied nanoflagellates showed measurable direct uptake of H-3-leucine, suggesting that the studied phototrophic protists were unable to utilize dissolved H-3-leucine at natural oceanic concentrations. More practically, the flow-sorting technique allowed rapid and unequivocal differentiation of organic nitrogen uptake between prokaryotic cells and eukaryotic cells in mixed microbial populations, reducing the need to establish and maintain axenic algal cultures.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | FEMS Microbiology Letters | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 0378-1097 | ||||
| Official Date: | September 2009 | ||||
| Dates: |
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| Volume: | Vol.298 | ||||
| Number: | No.2 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 166-173 | ||||
| DOI: | 10.1111/j.1574-6968.2009.01715.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Natural Environment Research Council (Great Britain) (NERC), National Oceanography Centre Core Programme | ||||
| Grant number: | NE/E016138/1 |
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