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Site-specific N-terminus conjugation of poly(mPEG(1100)) methacrylates to salmon calcitonin : synthesis and preliminary biological evaluation
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Sayers, Claire T., Mantovani, Giuseppe, Ryan, Sinead M., Randev, Rajan K., Keiper, Odin, Leszczyszyn, Oksana I., Blindauer, Claudia A., Brayden, David J. and Haddleton, David M.. (2009) Site-specific N-terminus conjugation of poly(mPEG(1100)) methacrylates to salmon calcitonin : synthesis and preliminary biological evaluation. Soft Matter, Vol.5 (No.16). pp. 3038-3046. ISSN 1744-683X
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Official URL: http://dx.doi.org/10.1039/b905335b
Abstract
Recent advances in polymerization strategies have led to the development of novel polymer (poly) peptide biohybrid materials with potential application in the field of macromolecular therapeutics. In this current work, comb-shaped alpha-aldehyde poly(monomethoxy polyethylene glycol)methacrylates (p(mPEG)MA) with molecular masses in the 6.5-109 kDa range were prepared and conjugated, via reductive amination, to the Cys1 N-terminus of salmon calcitonin (sCT), a calcitropic hormone currently administered for the treatment of a number of hypercalcaemia-related diseases. The conjugation site was determined by tryptic digestion of the sCT-p(mPEG(1100)) MA biohybrids in conjunction with LC-MS MALDI-TOF spectrometry. Preliminary in vitro biological tests show that the polymer conjugation does not interfere with the biological activity of the sCT.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
| Divisions: | Faculty of Science > Chemistry |
| Library of Congress Subject Headings (LCSH): | Bioconjugates, Polymerization, Macromolecules -- Therapeutic use |
| Journal or Publication Title: | Soft Matter |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 1744-683X |
| Date: | 2009 |
| Volume: | Vol.5 |
| Number: | No.16 |
| Number of Pages: | 9 |
| Page Range: | pp. 3038-3046 |
| Identification Number: | 10.1039/b905335b |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | University of Warwick, Engineering and Physical Sciences Research Council (EPSRC), Warwick Effect Polymers Ltd, Science Foundation Ireland |
| Grant number: | 04 IN3 B575 (SFI) |
| URI: | http://wrap.warwick.ac.uk/id/eprint/17496 |
Data sourced from Thomson Reuters' Web of Knowledge
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