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Genetically encoded imaging tools for investigating cell dynamics at a glance
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Sittewelle, Méghane, Ferrandiz, Nuria, Fesenko, Mary and Royle, Stephen J. (2023) Genetically encoded imaging tools for investigating cell dynamics at a glance. Journal of Cell Science, 136 (7). doi:10.1242/jcs.260783 ISSN 1477-9137.
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Official URL: https://doi.org/10.1242/jcs.260783
Abstract
The biology of a cell is the sum of many highly dynamic processes, each orchestrated by a plethora of proteins and other molecules. Microscopy is an invaluable approach to spatially and temporally dissect the molecular details of these processes. Hundreds of genetically encoded imaging tools have been developed that allow cell scientists to determine the function of a protein of interest in the context of these dynamic processes. Broadly, these tools fall into three strategies: observation, inhibition and activation. Using examples for each strategy, in this Cell Science at a Glance and the accompanying poster, we provide a guide to using these tools to dissect protein function in a given cellular process. Our focus here is on tools that allow rapid modification of proteins of interest and how observing the resulting changes in cell states is key to unlocking dynamic cell processes. The aim is to inspire the reader's next set of imaging experiments.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cytology, Microscopy, Imaging systems in biology, Molecular biology, Neural transmission, Optogenetics | ||||||||||||||||||
Journal or Publication Title: | Journal of Cell Science | ||||||||||||||||||
Publisher: | The Company of Biologists | ||||||||||||||||||
ISSN: | 1477-9137 | ||||||||||||||||||
Official Date: | April 2023 | ||||||||||||||||||
Dates: |
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Volume: | 136 | ||||||||||||||||||
Number: | 7 | ||||||||||||||||||
DOI: | 10.1242/jcs.260783 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
Date of first compliant deposit: | 11 September 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 11 April 2024 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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