Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage phi M1
UNSPECIFIED. (1997) Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage phi M1. MICROBIOLOGY-UK, 143 (Part 7). pp. 2433-2438. ISSN 1350-0872Full text not available from this repository.
Using enrichment methods, a new bacteriophage (phi M1) was isolated, which is capable of generalized transduction in Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043. phi M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. phi M1 infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates and for the transductional transfer of selectable markers between strains.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||MICROBIOLOGY-UK|
|Publisher:||SOC GENERAL MICROBIOLOGY|
|Number of Pages:||6|
|Page Range:||pp. 2433-2438|
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