UNSPECIFIED (1997) Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage phi M1. MICROBIOLOGY-UK, 143 (Part 7). pp. 2433-2438. ISSN 1350-0872

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Abstract

Using enrichment methods, a new bacteriophage (phi M1) was isolated, which is capable of generalized transduction in Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043. phi M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. phi M1 infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates and for the transductional transfer of selectable markers between strains.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	MICROBIOLOGY-UK
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	1350-0872
Date:	July 1997
Volume:	143
Number:	Part 7
Number of Pages:	6
Page Range:	pp. 2433-2438
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/17552

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