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An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton
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UNSPECIFIED (1997) An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 63 (6). pp. 2411-2420. ISSN 0099-2240
Full text not available from this repository.Abstract
In the marine cyanobacterium Synechococcus sp. strain WH7803, PstS is a 32-kDa cell wall-associated phosphate-binding protein specifically synthesized under conditions of restricted inorganic phosphate (P-i) availability (D. J. Scanlan, N. H. Mann, and N. G. Carr, Mol; Microbiol. 10:181-191, 1993). We have assessed its use as a potential diagnostic marker for the P status of photosynthetic picoplankton. Expression of PstS in Synechococcus sp, strain WH7803 was observed when the P-i concentration fell below 50 nM, demonstrating that the protein is induced at concentrations of P-i typical of oligotrophic conditions. PstS expression could be specifically detected by use of standard Western blotting (immunoblotting) techniques in natural mesocosm samples under conditions in which the N/P ratio was artificially manipulated to force P depletion. In addition, we have developed an immunofluorescence assay that can detect PstS expression in single Synechococcus cells both in laboratory cultures and natural samples, We show that antibodies raised against PstS cross-react with P-depleted Prochlorococcus cells, extending the use of these antibodies to both major groups of prokaryotic photosynthetic picoplankton. Furthermore, DNA sequencing of a Prochlorococcus pstS homolog demonstrated high amino acid sequence identity (77%) with the marine Synechococcus sp. strain WH7803 protein, including those residues in Escherichia coil PstS known to be directly involved in phosphate binding.
| Item Type: | Journal Article |
|---|---|
| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
| Journal or Publication Title: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
| Publisher: | AMER SOC MICROBIOLOGY |
| ISSN: | 0099-2240 |
| Date: | June 1997 |
| Volume: | 63 |
| Number: | 6 |
| Number of Pages: | 10 |
| Page Range: | pp. 2411-2420 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/17743 |
Data sourced from Thomson Reuters' Web of Knowledge
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