UNSPECIFIED (1997) Comparison of ribosome-inactivating proteins in the induction of apoptosis. TOXICOLOGY LETTERS, 91 (2). pp. 121-127. ISSN 0378-4274

Full text not available from this repository.

Abstract

The aim of this study was to evaluate the ability of verocytotoxin-l (VT1), VT1 B chain alone, ricin and a hybrid toxin (RASTA2) consisting of ricin A chain linked to VT1 B chain to inhibit protein synthesis and to induce apoptosis. The lethal effects of the toxins were compared using vero cells (originating from green African monkey kidney tissue). As previously described cell death occurred through apoptosis which was quantified using the diphenylamine assay. DNA fragmentation was seen with VT1 at 10 pg/ml but there was no effect with B chain alone. Fragmentation with ricin was seen at 10 ng/ml and with RASTA2 at 1 ng/ml. Protein synthesis inhibition was measured by [S-35]methionine incorporation. VT1 had an IC50 of 0.0024 ng/ml, B chain alone was ineffective at inhibiting protein synthesis. Ricin had an IC50 of 0.39 ng/ml and RASTA2 of 1.7 ng/ml. In vero cells the B chain of these toxins does not participate in cell killing. (C) 1997 Elsevier Science Ireland Ltd.

Item Type:	Journal Article
Subjects:	R Medicine > RA Public aspects of medicine
Journal or Publication Title:	TOXICOLOGY LETTERS
Publisher:	ELSEVIER SCI IRELAND LTD
ISSN:	0378-4274
Date:	28 April 1997
Volume:	91
Number:	2
Number of Pages:	7
Page Range:	pp. 121-127
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/17744

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item