Solvent effect on enzyme-catalyzed synthesis of beta-D-glucosides using the reverse hydrolysis method: Application to the preparative-scale synthesis of 2-hydroxybenzyl and octyl beta-D-glucopyranosides
UNSPECIFIED. (1997) Solvent effect on enzyme-catalyzed synthesis of beta-D-glucosides using the reverse hydrolysis method: Application to the preparative-scale synthesis of 2-hydroxybenzyl and octyl beta-D-glucopyranosides. ENZYME AND MICROBIAL TECHNOLOGY, 20 (8). pp. 597-603. ISSN 0141-0229Full text not available from this repository.
Almond beta-D-glucosidase was used to catalyze alkyl-beta-D-glucoside synthesis by reacting glucose and the alcohol in organic media. The influence of five different solvents and the thermodynamic water activity on the reaction have been studied. The best yields were obtained in 80 or 90% (v/v) tert-butanol, acetone, or acetonitrile where the enzyme is very stable. In this enzymatic synthesis under thermodynamic control, the yield increases as the water activity of the reaction medium decreases. Enzymatic preparative-scale syntheses were performed in a tert-butanol-water mixture which was found to be the most appropriate medium. 2-Hydroxybenzyl beta-D-glucopyranoside was obtained in 17% yield using a 90:10 (v/v) tert-butanol-water mixture. Octyl-beta-glucopyranoside was obtained in 8% yield using a 60:30:10 (v/v) tert-butanol-octanol-water mixture. (C) 1997 by Elsevier Science Inc.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology|
|Journal or Publication Title:||ENZYME AND MICROBIAL TECHNOLOGY|
|Publisher:||ELSEVIER SCIENCE INC|
|Number of Pages:||7|
|Page Range:||pp. 597-603|
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