UNSPECIFIED (1997) The soluble methane monooxygenase gene cluster of the trichloroethylene-degrading methanotroph Methylocystis sp. strain M. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 63 (5). pp. 1898-1904. ISSN 0099-2240

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Abstract

In methanotrophic bacteria, methane is oxidized to methanol by the enzyme methane monooxygenase (MMO), The soluble MMO enzyme complex from Methylocystis sp. strain M also oxidizes a wide range of aliphatic and aromatic compounds, including trichloroethylene. In this study, heterologous DNA probes from the type II methanotroph Methylosinus trichosporium OE3b were used to isolate soluble MMO (sMMO) genes from the type IP methanotroph Methylocystis sp. strain M. sMMO genes from strain M are clustered on the chromosome and show a high degree of identity with the corresponding genes from Methylosinus trichosporium OB3b. Sequencing and phylogenetic analysis of the 16S rRNA gene from Methylocystis sp. strain M have confirmed that it is most closely related to the type II methanotroph Methylocystis parvus OBBP, which, unlike Methylocystis sp. strain M, does not possess an sMMO. A similar phylogenetic analysis using the pmoA gene, which encodes the 27-kDa polypeptide of the particulate MMO, also places Methylocystis sp. strain M firmly in the genus Methylocystis. This is the first report of isolation and characterization of methane oxidation genes from methanotrophs of the genus methylocystis.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0099-2240
Date:	May 1997
Volume:	63
Number:	5
Number of Pages:	7
Page Range:	pp. 1898-1904
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/17798

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