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Mapping of the target antigens of the rotavirus-specific cytotoxic T cell response
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UNSPECIFIED (1997) Mapping of the target antigens of the rotavirus-specific cytotoxic T cell response. JOURNAL OF GENERAL VIROLOGY, 78 (Part 5). pp. 1065-1075.
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Abstract
The cytotoxic T cell (CTL) response in C57/Bl6 (H-2(b)) mice to rotavirus has been analysed using a cognate set of vaccinia virus recombinants covering the 12 primary gene products of the UKtc strain of bovine rotavirus. The gene products of RNA segments 5 (VP5/NSP-1) and 8 (VP7) both elicited a classic CD8(+) Class I MHC restricted CTL response. Using L cells transfected with specific Class I MHC loci as targets the VP5/NSP-1 response was found to be restricted at D-b and the VP7 response at K-b. Vaccinia virus recombinants expressing VP7 genes from seven G serotypes were used to show that the CTL response to this antigen is completely cross-reactive. By contrast, using the same strategy the CTL response to VP5/NSP-1 was found to be virus strain specific. A vaccinia virus recombinant carrying RNA segment 5 from the deletion mutant P9D Delta 5 was used to localize at least one CTL epitope in VP5/NSP-1 to the first 150 amino acids of the protein. The expression of a number of fragments of VP7 in vaccinia virus recombinants was used to show that the CTL epitope (amino acids 31-40) previously identified through the use of synthetic peptides is virus serotype specific rather than cross-reactive.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF GENERAL VIROLOGY | ||||
| Publisher: | SOC GENERAL MICROBIOLOGY | ||||
| ISSN: | 0022-1317 | ||||
| Official Date: | May 1997 | ||||
| Dates: |
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| Volume: | 78 | ||||
| Number: | Part 5 | ||||
| Number of Pages: | 11 | ||||
| Page Range: | pp. 1065-1075 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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