UNSPECIFIED. (1997) Molecular detection of streptomycin-producing streptomycetes in Brazilian soils. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 63 (4). pp. 1288-1297. ISSN 0099-2240

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Abstract

Actinomycetes were isolated from soybean rhizosphere soil collected at two field sites in Brazil, All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains were streptomycin resistant. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains fi om cultivated sites being placed in cluster 1, Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin-producing streptomycetes were phylogenetically diverse, It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0099-2240
Date:	April 1997
Volume:	63
Number:	4
Number of Pages:	10
Page Range:	pp. 1288-1297
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/17883

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