The Library
Molecular detection of streptomycin-producing streptomycetes in Brazilian soils
Tools
UNSPECIFIED (1997) Molecular detection of streptomycin-producing streptomycetes in Brazilian soils. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 63 (4). pp. 1288-1297. ISSN 0099-2240.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Abstract
Actinomycetes were isolated from soybean rhizosphere soil collected at two field sites in Brazil, All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains were streptomycin resistant. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains fi om cultivated sites being placed in cluster 1, Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin-producing streptomycetes were phylogenetically diverse, It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
||||
Journal or Publication Title: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | ||||
Publisher: | AMER SOC MICROBIOLOGY | ||||
ISSN: | 0099-2240 | ||||
Official Date: | April 1997 | ||||
Dates: |
|
||||
Volume: | 63 | ||||
Number: | 4 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 1288-1297 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |