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The protozoan trichomonas vaginalis targets bacteria with laterally acquired NlpC/P60 peptidoglycan hydrolases
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Pinheiro, Jully, Biboy, Jacob, Vollmer, Waldemar, Hirt, Robert P., Keown, Jeremy R., Artuyants, Anastasiia, Black, Moyra M., Goldstone, David C., Simoes-Barbosa, Augusto and Johnson, Patricia J. (2018) The protozoan trichomonas vaginalis targets bacteria with laterally acquired NlpC/P60 peptidoglycan hydrolases. mBio, 9 (6). e01784-18. doi:10.1128/mBio.01784-18 ISSN 2150-7511.
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Official URL: http://doi.org/10.1128/mBio.01784-18
Abstract
The human eukaryotic pathogen Trichomonas vaginalis causes trichomoniasis, a prevalent sexually transmitted infection. This extracellular protozoan is intimately associated with the human vaginal mucosa and microbiota, but key aspects of the complex interactions between the parasite and the vaginal bacteria remain elusive. We report that T. vaginalis has acquired, by lateral gene transfer from bacteria, genes encoding peptidoglycan hydrolases of the NlpC/P60 family. Two of the T. vaginalis enzymes were active against bacterial peptidoglycan, retaining the active-site fold and specificity as dl-endopeptidases. The endogenous NlpC/P60 genes are transcriptionally upregulated in T. vaginalis in the presence of bacteria. The overexpression of an exogenous copy enables the parasite to outcompete bacteria from mixed cultures, consistent with the biochemical activity of the enzyme. Our study results highlight the relevance of the interactions of this eukaryotic pathogen with bacteria, a poorly understood aspect of the biology of this important human parasite.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | mBio | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 2150-7511 | ||||
Official Date: | 11 December 2018 | ||||
Dates: |
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Volume: | 9 | ||||
Number: | 6 | ||||
Article Number: | e01784-18 | ||||
DOI: | 10.1128/mBio.01784-18 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
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