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Small oligomers of ribulose-bisphosphate carboxylase/oxygenase (Rubisco) Activase are required for biological activity
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Keown, Jeremy R., Griffin, Michael D. W., Mertens, Haydyn D. T. and Pearce, F. Grant (2013) Small oligomers of ribulose-bisphosphate carboxylase/oxygenase (Rubisco) Activase are required for biological activity. Journal of Biological Chemistry, 288 (28). pp. 20607-20615. doi:10.1074/jbc.M113.466383 ISSN 0021-9258.
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Official URL: http://doi.org/10.1074/jbc.M113.466383
Abstract
Ribulose-bisphosphate carboxylase/oxygenase (Rubisco) activase uses the energy from ATP hydrolysis to remove tight binding inhibitors from Rubisco, thus playing a key role in regulating photosynthesis in plants. Although several structures have recently added much needed structural information for different Rubisco activase enzymes, the arrangement of these subunits in solution remains unclear. In this study, we use a variety of techniques to show that Rubisco activase forms a wide range of structures in solution, ranging from monomers to much higher order species, and that the distribution of these species is highly dependent on protein concentration. The data support a model in which Rubisco activase forms an open spiraling structure rather than a closed hexameric structure. At protein concentrations of 1 μm, corresponding to the maximal activity of the enzyme, Rubisco activase has an oligomeric state of 2–4 subunits. We propose a model in which Rubisco activase requires at least 1 neighboring subunit for hydrolysis of ATP.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Journal of Biological Chemistry | ||||
Publisher: | American Society for Biochemistry and Molecular Biology | ||||
ISSN: | 0021-9258 | ||||
Official Date: | July 2013 | ||||
Dates: |
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Volume: | 288 | ||||
Number: | 28 | ||||
Page Range: | pp. 20607-20615 | ||||
DOI: | 10.1074/jbc.M113.466383 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
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