UNSPECIFIED (1997) Comparison of novel sampling methods for the analysis of capillary electrophoresis fractions by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In: 22nd Annual British Mass Spectrometry Conference, SEP 08-11, 1996, SWANSEA, WALES.

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Abstract

Two methods for the collection of capillary electrophoresis fractions for analysis by matrix-assisted laser desorption/ionization have been investigated; the first using a novel ion-porous Joint design for dropwise fraction collection and the second, a process of electrospray deposition, Sensitivity shot-to-shot reproducibility, effect of matrix preparation and influence of buffer additives encountered in micellar electrokinetic capillary chromatography (MECC) have been examined using a standard peptide, methionine enkephalin. The direct collection of droplets from the capillary outlet was found to give a superior signal intensity, whereas the electrospray method, in its first stage of development, suffered through dilution effects and excessively wide sample distribution. The target compound was successfully collected by electrospray deposition and results indicated that signal intensity and sample homogeneity would be enhanced if the sprayed output could be collected into the sample wells in its entirety, The advantage of using electrospray deposition for the preparation of matrix layers followed by direct droplet collection onto the electrosprayed matrix was clearly demonstrated by improvements in both sensitivity and shot-to-shot variance In signal intensity across the sample well compared with collection onto spotted matrix, In addition, compatibility of the droplet collection method with MECC was illustrated by the recovery of the compound of interest from a buffer containing 30 mM sodium dodecyl sulphate. (C) 1997 by John Wiley & Sons, Ltd.

Item Type:	Conference Item (UNSPECIFIED)
Subjects:	Q Science > QD Chemistry Q Science > QC Physics
Journal or Publication Title:	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Publisher:	JOHN WILEY & SONS LTD
ISSN:	0951-4198
Date:	1997
Volume:	11
Number:	2
Number of Pages:	5
Page Range:	pp. 214-218
Publication Status:	Published
Title of Event:	22nd Annual British Mass Spectrometry Conference
Location of Event:	SWANSEA, WALES
Date(s) of Event:	SEP 08-11, 1996
URI:	http://wrap.warwick.ac.uk/id/eprint/18008

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