Free ricin A chain reaches an early compartment of the secretory pathway before it enters the cytosol
UNSPECIFIED. (1996) Free ricin A chain reaches an early compartment of the secretory pathway before it enters the cytosol. EXPERIMENTAL CELL RESEARCH, 229 (2). pp. 447-451. ISSN 0014-4827Full text not available from this repository.
During the intoxication of mammalian cells by ricin, the catalytically active A chain must cross the membrane of an intracellular compartment in order to reach its ribosomal substrates in the cytosol. The actual site of ricin A chain translocation is unclear, and conflicting views hold that it enters the cytosol from endosomes or from an early compartment of the secretory pathway, possibly the lumen of the endoplasmic reticulum. Here we show that treating cells with brefeldin A, or transiently overexpressing mutant GTPases known to inhibit biochemical complexes mediating anterograde and retrograde transport between the endoplasmic reticulum and the Golgi complex, protected cells from intoxication by free ricin A chain. These data indicate that ricin A chain, either free or as part of intact ricin, reaches an early compartment of the secretory pathway before translocation into the cytosol occurs. (C) 1996 Academic Press, Inc.
|Item Type:||Journal Article|
|Subjects:||R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||EXPERIMENTAL CELL RESEARCH|
|Publisher:||ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS|
|Official Date:||15 December 1996|
|Number of Pages:||5|
|Page Range:||pp. 447-451|
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