UNSPECIFIED. (1996) Free ricin A chain reaches an early compartment of the secretory pathway before it enters the cytosol. EXPERIMENTAL CELL RESEARCH, 229 (2). pp. 447-451. ISSN 0014-4827

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Abstract

During the intoxication of mammalian cells by ricin, the catalytically active A chain must cross the membrane of an intracellular compartment in order to reach its ribosomal substrates in the cytosol. The actual site of ricin A chain translocation is unclear, and conflicting views hold that it enters the cytosol from endosomes or from an early compartment of the secretory pathway, possibly the lumen of the endoplasmic reticulum. Here we show that treating cells with brefeldin A, or transiently overexpressing mutant GTPases known to inhibit biochemical complexes mediating anterograde and retrograde transport between the endoplasmic reticulum and the Golgi complex, protected cells from intoxication by free ricin A chain. These data indicate that ricin A chain, either free or as part of intact ricin, reaches an early compartment of the secretory pathway before translocation into the cytosol occurs. (C) 1996 Academic Press, Inc.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	EXPERIMENTAL CELL RESEARCH
Publisher:	ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
ISSN:	0014-4827
Date:	15 December 1996
Volume:	229
Number:	2
Number of Pages:	5
Page Range:	pp. 447-451
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/18060

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