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A human CD4(+) T-cell line expresses functional CD64 (Fc gamma RI), CD32 (Fc gamma RII), and CD16 (Fc gamma RIII) receptors but these do not enhance the infectivity of HIV-1-IgG complexes
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UNSPECIFIED (1997) A human CD4(+) T-cell line expresses functional CD64 (Fc gamma RI), CD32 (Fc gamma RII), and CD16 (Fc gamma RIII) receptors but these do not enhance the infectivity of HIV-1-IgG complexes. IMMUNOLOGY, 90 (1). pp. 109-114. ISSN 0019-2805.
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Abstract
T cells do not generally express Fc receptors (FcRs). However, we report here that C8166 cells, a human CD4(+) T lymphoblastoid cell line, widely used in research into the human immunodeficiency virus type 1 (HIV-1), expressed CD64 (Fc gamma RI), CD32 (Fc gamma RII), and CD16 (Fc gamma RIII) on the plasma membrane as shown by immunostaining with specific monoclonal antibody fragments. Another human CD4(+) T lymphoblastoid cell line, H9, expressed none of these FcRs. C8166 cells bound monomeric normal rat serum IgG in a dose-dependent manner, and when saturated bound heat-complexed immunoglobulin G (IgG) also dose dependently. These observations are consistent with the presence on the C8166 T-cell line of both high- and low-affinity Fc gamma Rs. Fc gamma Rs are putative receptors for virus-IgG complexes, but in this study did not enhance infectivity of HIV-1 complexed with a human neutralizing mAb or three rat neutralizing mAbs. Virus complexed with a non-neutralizing mouse mAb was unable to infect cells using Fc gamma Rs as receptors after CD4 was blocked with a specific anti-CD4 mAb.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology > QR180 Immunology | ||||
Journal or Publication Title: | IMMUNOLOGY | ||||
Publisher: | BLACKWELL SCIENCE LTD | ||||
ISSN: | 0019-2805 | ||||
Official Date: | January 1997 | ||||
Dates: |
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Volume: | 90 | ||||
Number: | 1 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 109-114 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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