Molecular analysis of enrichment cultures of marine methane oxidising bacteria
UNSPECIFIED (1996) Molecular analysis of enrichment cultures of marine methane oxidising bacteria. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 203 (1). pp. 27-38. ISSN 0022-0981Full text not available from this repository.
Methane oxidising bacteria (methanotrophs) are an important group of bacteria in the global cycling of methane and may act as a sink for methane in freshwater, soil and marine environments. Their ecology is fairly well documented in terrestrial and freshwater environments but marine methanotrophs have been less-well characterised, partly due to the difficulties in the isolation, cultivation and identification of these organisms. Molecular ecological techniques are being developed to aid the characterisation and enumeration of marine methane oxidising bacteria and to assess their role in the turn-over of methane in the marine environment. 16S ribosomal RNA gene probes have been developed to detect and identify marine methanotrophs. The use of these probes, coupled with fluorescence microscopy techniques, to aid enrichment, cultivation and subsequent characterisation of marine methanotrophs is described. The use of the polymerase chain reaction to amplify methane monooxygenase genes from enrichments of marine methanotrophs is also described.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Journal or Publication Title:||JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY|
|Publisher:||ELSEVIER SCIENCE BV|
|Date:||1 October 1996|
|Number of Pages:||12|
|Page Range:||pp. 27-38|
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