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Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography
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Bauda, Elda, Gallet, Benoit, Moravcova, Jana, Effantin, Gregory, Chan, Helena, Novacek, Jiri, Jouneau, Pierre-Henri, Rodrigues, Christopher D. A., Schoehn, Guy, Moriscot, Christine and Morlot, Cecile (2024) Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography. Nature Communications, 15 (1). 1376. doi:10.1038/s41467-024-45770-6 ISSN 2041-1723.
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Official URL: http://doi.org/10.1038/s41467-024-45770-6
Abstract
Bacterial spores owe their incredible resistance capacities to molecular structures that protect the cell content from external aggressions. Among the determinants of resistance are the quaternary structure of the chromosome and an extracellular shell made of proteinaceous layers (the coat), the assembly of which remains poorly understood. Here, in situ cryo-electron tomography on lamellae generated by cryo-focused ion beam micromachining provides insights into the ultrastructural organization of Bacillus subtilis sporangia. The reconstructed tomograms reveal that early during sporulation, the chromosome in the forespore adopts a toroidal structure harboring 5.5-nm thick fibers. At the same stage, coat proteins at the surface of the forespore form a stack of amorphous or structured layers with distinct electron density, dimensions and organization. By analyzing mutant strains using cryo-electron tomography and transmission electron microscopy on resin sections, we distinguish seven nascent coat regions with different molecular properties, and propose a model for the contribution of coat morphogenetic proteins.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Journal or Publication Title: | Nature Communications | ||||||
Publisher: | Nature Publishing Group UK | ||||||
ISSN: | 2041-1723 | ||||||
Official Date: | 14 February 2024 | ||||||
Dates: |
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Volume: | 15 | ||||||
Number: | 1 | ||||||
Article Number: | 1376 | ||||||
DOI: | 10.1038/s41467-024-45770-6 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 19 March 2024 | ||||||
Date of first compliant Open Access: | 19 March 2024 |
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