DAG, a gene required for chloroplast differentiation and palisade development in Antirrhinum majus
UNSPECIFIED (1996) DAG, a gene required for chloroplast differentiation and palisade development in Antirrhinum majus. EMBO JOURNAL, 15 (16). pp. 4194-4207. ISSN 0261-4189Full text not available from this repository.
We have identified a mutation at the DAG locus of Antirrhinum majus which blocks the development of chloroplasts to give white leaves with green revertant sectors, The green areas contain normal chloroplasts whereas the white areas have small plastids that resemble proplastids, The cotyledons of dark-grown dag mutant seedlings have plastids which also resemble proplastids, The palisade cells in the white areas of dag mutant leaves also lack their characteristic columnar shape, The DAG locus was cloned by transposon tagging: DAG encodes a novel protein with a predicted M(r) of 26k, which is targeted to the plastids, Cleavage of its predicted transit peptide gives a mature protein of M(r) 20k, Screening of databases and analysis of Southern blots gave evidence that DAG belongs to a protein family with homology to several proteins of unknown function from plants, Expression of DAG is required for expression of nuclear genes affecting the chloroplasts, such as CAB and RBCS, and also for expression of the plastidial gene RPOB encoding the plastidial RNA polymerase beta subunit, indicating that it functions very early in chloroplast development.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||EMBO JOURNAL|
|Publisher:||OXFORD UNIV PRESS UNITED KINGDOM|
|Date:||15 August 1996|
|Number of Pages:||14|
|Page Range:||pp. 4194-4207|
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