UNSPECIFIED (1996) An Arabidopsis thaliana cDNA encoding PS II-X, a 4.1 kDa component of photosystem II: A bipartite presequence mediates SecA/Delta pH-independent targeting into thylakoids. FEBS LETTERS, 390 (2). pp. 175-178. ISSN 0014-5793

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Abstract

Higher plant photosystem II preparations contain a 4.1 kDa polypeptide (subunit X) associated with the oxygen-evolving core complex, We describe the isolation of a cDNA encoding PS II-X from Arabidopsis thaliana, in which the C-terminal region is highly homologous to partially sequenced PS II-X from wheat and spinach, The mature protein of 42 residues is preceded by a 74-residue, bipartite presequence similar to those involved in the targeting of nuclear-encoded thylakoid lumen proteins, although hydrophobicity analysis indicates the presence of a single transmembrane span in the mature protein, Moreover, import of pre-PS II-X into the thylakoid membrane of isolated chloroplasts is unaffected by inhibitors of either the Sec- or Delta pH-dependent thylakoidal protein translocases, suggesting a spontaneous insertion mechanism, PS II-X appears to be encoded as a mature protein by the plastid genome in the chlorophyll a+c-containing alga, Odontella sinensis. We thus propose that the thylakoid transfer signal of Arabidopsis pre-PS II-X represents a recent acquisition, in phylogenetic terms, compared with signals of Sec-dependent lumenal proteins.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	FEBS LETTERS
Publisher:	ELSEVIER SCIENCE BV
ISSN:	0014-5793
Date:	22 July 1996
Volume:	390
Number:	2
Number of Pages:	4
Page Range:	pp. 175-178
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/18538

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