Xu, Yunhe, Mirmalek-Sanil, Sayed-Hadi, Lin, Feng, Zhang, Junlong and Oreffo, Richard O.C. (2007) Adipocyte differentiation induced using non-specific siRNA controls in cultured human mesenchymal stem cells. RNA, Vol.13 (No.8). pp. 1179-1183. ISSN 1355-1183

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Abstract

RNA interference (RNAi) is gene silencing induced by double-stranded RNA of 21-23 nucleotides in length, termed small interfering RNA, or siRNA. RNAi-based techniques have been widely applied to elucidate gene function, identify drug targets and used in trials as a promising adjunct to silence disease-causing genes. However, emerging evidence suggests unexpected changes in expression of untargeted genes as a consequence of an off-target effect by RNAi in mammalian cells. To date, our understanding of such effects on stem cells is limited. We transfected human fetal femur-derived mesenchymal stem cells using commercially available non-specific siRNA controls and examined adipocyte differentiation in the cells using morphology, histochemistry and quantitative real-time PCR to examine the expression of key genes for adipogenic or osteogenic differentiation. We report here the induction of adipocyte differentiation in human mesenchymal stem cells using non-specific siRNAs raising concerns as to the specificity of RNAi in stem cells and, critically, a need to understand and delineate the rules governing the specificity of RNAi.

Item Type:	Journal Item
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Small interfering RNA, RNA, Fat cells
Journal or Publication Title:	RNA
Publisher:	Cold Spring Harbor Laboratory Press
ISSN:	1355-1183
Date:	2 May 2007
Volume:	Vol.13
Number:	No.8
Page Range:	pp. 1179-1183
Identification Number:	10.1261/rna.527207
Status:	Not Peer Reviewed
Access rights to Published version:	Open Access
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URI:	http://wrap.warwick.ac.uk/id/eprint/186

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