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Adipocyte differentiation induced using non-specific siRNA controls in cultured human mesenchymal stem cells
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Xu, Yunhe, Mirmalek-Sanil, Sayed-Hadi, Lin, Feng, Zhang, Junlong and Oreffo, Richard O.C. (2007) Adipocyte differentiation induced using non-specific siRNA controls in cultured human mesenchymal stem cells. RNA, Vol.13 (No.8). pp. 1179-1183. doi:10.1261/rna.527207
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Official URL: http://dx.doi.org/10.1261/rna.527207
Abstract
RNA interference (RNAi) is gene silencing induced by double-stranded RNA of 21-23
nucleotides in length, termed small interfering RNA, or siRNA. RNAi-based techniques have
been widely applied to elucidate gene function, identify drug targets and used in trials as a
promising adjunct to silence disease-causing genes. However, emerging evidence suggests
unexpected changes in expression of untargeted genes as a consequence of an off-target effect
by RNAi in mammalian cells. To date, our understanding of such effects on stem cells is
limited. We transfected human fetal femur-derived mesenchymal stem cells using
commercially available non-specific siRNA controls and examined adipocyte differentiation
in the cells using morphology, histochemistry and quantitative real-time PCR to examine the
expression of key genes for adipogenic or osteogenic differentiation. We report here the
induction of adipocyte differentiation in human mesenchymal stem cells using non-specific
siRNAs raising concerns as to the specificity of RNAi in stem cells and, critically, a need to
understand and delineate the rules governing the specificity of RNAi.
Item Type: | Journal Item | ||||
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Subjects: | R Medicine > R Medicine (General) | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Library of Congress Subject Headings (LCSH): | Small interfering RNA, RNA, Fat cells | ||||
Journal or Publication Title: | RNA | ||||
Publisher: | Cold Spring Harbor Laboratory Press | ||||
ISSN: | 1355-1183 | ||||
Official Date: | 2 May 2007 | ||||
Dates: |
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Volume: | Vol.13 | ||||
Number: | No.8 | ||||
Page Range: | pp. 1179-1183 | ||||
DOI: | 10.1261/rna.527207 | ||||
Status: | Not Peer Reviewed | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 1 January 2016 |
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