UNSPECIFIED (1996) Regulation of bacterial methane oxidation: Transcription of the soluble methane monooxygenase operon of Methylococcus capsulatus (Bath) is repressed by copper ions. MICROBIOLOGY-UK, 142 (Part 5). pp. 1289-1296. ISSN 1350-0872

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Abstract

Methane is oxidized to methanol by the enzyme methane mono-oxygenase (MMO) in methanotrophic bacteria. In previous work, this multicomponent enzyme system has been extensively characterized at the biochemical and molecular level. Copper ions have been shown to irreversibly inhibit MMO activity in vivo and in vitro, but the effect of copper ions on transcription of the genes encoding the soluble (cytoplasmic) MMO (sMMO) has not previously been investigated. To examine more closely the regulation of bacterial methane oxidation and to determine the role of copper in this process, we have investigated transcriptional regulation of the sMMO gene cluster in the methanotrophic bacterium Methylococcus capsulatus (Bath). Using Northern blot analysis and primer extension experiments, it was shown that the six ORFs of the sMMO gene cluster are organized as an operon and the transcripts produced upon expression of this operon have been identified. The synthesis of these transcripts was under control of a single copper-regulated promoter, which is as yet not precisely defined.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	MICROBIOLOGY-UK
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	1350-0872
Date:	May 1996
Volume:	142
Number:	Part 5
Number of Pages:	8
Page Range:	pp. 1289-1296
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/18824

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