UNSPECIFIED. (1996) Accessory cell requirements for T lymphocyte activation and interferon-gamma production in peripheral lymph nodes. IMMUNOLOGY LETTERS, 49 (1-2). pp. 49-55. ISSN 0165-2478

Abstract

Allergen-activated draining lymph node cells (LNC) isolated from mice exposed topically to the contact allergen oxazolone mount vigorous proliferative responses and secrete substantial amounts of interferon-gamma (IFN-gamma) when cultured with the T lymphocyte mitogen concanavalin A (con A). In contrast, although naive LNC prepared from untreated mice display con A-driven proliferative responses of comparable magnitude, they produce only very low levels of IFN-gamma. Secretion of IFN-gamma by con A-stimulated naive LNC was augmented significantly by the addition to culture of a small number of syngeneic dendritic cells (DC), under conditions where there was no influence on the vigour of proliferative responses and where the exogenous DC themselves failed to produce IFN-gamma. Augmentation of IFN-gamma production was not observed when exogenous populations depleted of DC were added to culture. It is proposed that discrete aspects of the primary activation of naive T lymphocytes display differential requirements for accessory cells and that the development of IFN-gamma producing cells necessitates sufficient numbers of dendritic cells.

Data sourced from Thomson Reuters' Web of Knowledge

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