The intracellular transport of ricin: Why mammalian cells are killed and how Ricinus cells survive
UNSPECIFIED (1996) The intracellular transport of ricin: Why mammalian cells are killed and how Ricinus cells survive. In: SFPV Meeting on Targeting and Glycosylation of Plant Secretory Proteins, BOIS GUILLAUME, FRANCE, MAR 31, 1995. Published in: PLANT PHYSIOLOGY AND BIOCHEMISTRY, 34 (2). pp. 253-261.Full text not available from this repository.
Ricin is an abundant protein present in Ricinus communis seeds (castor bean) that is potently toxic to mammalian cells. It is a heterodimeric protein consisting of an enzymic polypeptide that catalyses the N-glycosidic cleavage of a specific adenine residue from 28S ribosomal RNA, covalently joined by a single disulphide bond to a galactose-binding lectin. The enzymatic activity renders ribosomes containing depurinated 28S RNA incapable of protein synthesis. The bipartite structure and functional properties of ricin allow it to bind to galactosides on the cell surface, enter the cell via endocytic uptake, and traverse an intracellular membrane to deliver a catalytically-active polypeptide into the cell cytosol where it irreversibly inhibits protein synthesis to cause the death of the cell. Ricinus ribosomes which synthesise ricin are also susceptible to the catalytic action of this protein, and the strategy the plant uses to avoid suicide during ricin biosynthesis is discussed.
|Item Type:||Conference Item (UNSPECIFIED)|
|Subjects:||S Agriculture > SB Plant culture|
|Journal or Publication Title:||PLANT PHYSIOLOGY AND BIOCHEMISTRY|
|Number of Pages:||9|
|Page Range:||pp. 253-261|
|Title of Event:||SFPV Meeting on Targeting and Glycosylation of Plant Secretory Proteins|
|Location of Event:||BOIS GUILLAUME, FRANCE|
|Date(s) of Event:||MAR 31, 1995|
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