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High degree of genetic variation in Prochlorococcus (Prochlorophyta) revealed by RFLP analysis

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UNSPECIFIED (1996) High degree of genetic variation in Prochlorococcus (Prochlorophyta) revealed by RFLP analysis. EUROPEAN JOURNAL OF PHYCOLOGY, 31 (1). pp. 1-9. ISSN 0967-0262

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Abstract

A genetic characterization of nine strains of Prochlorococcus originating from various depths of the Mediterranean Sea, the Sargasso Sea, the North and the tropical Atlantic Ocean and the Pacific Ocean was performed by restriction fragment length polymorphism (RFLP) mapping using probes for rbcL, rbcS, psbA and woxA. This study revealed extensive genetic variation among strains, which were grouped into two distinct clusters. Unexpectedly, with one exception (TATL1 strain), the strains clustered by isolation depth (i.e. near surface versus deep isolates) rather than by geographic origin. These relationships were confirmed by secondary RFLP analysis of psbA amplification products using Prochlorococcus-specific polymerase chain reaction (PCR) primers.

Item Type: Journal Article
Subjects: S Agriculture > SB Plant culture
Q Science > QH Natural history > QH301 Biology
Journal or Publication Title: EUROPEAN JOURNAL OF PHYCOLOGY
Publisher: CAMBRIDGE UNIV PRESS
ISSN: 0967-0262
Date: February 1996
Volume: 31
Number: 1
Number of Pages: 9
Page Range: pp. 1-9
Publication Status: Published
URI: http://wrap.warwick.ac.uk/id/eprint/18985

Data sourced from Thomson Reuters' Web of Knowledge

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