UNSPECIFIED (1996) Alkaline phosphatase activity of Escherichia coli starved in sterile lake water microcosms. JOURNAL OF APPLIED BACTERIOLOGY, 80 (3). pp. 252-258. ISSN 0021-8847

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Abstract

Escherichia coli grown in high or low phosphate medium was inoculated into a lake water starvation medium. The viable count decreased at 37 degrees C but not at the lower temperatures over 70 d. Alkaline phosphatase was monitored using a colorimetric assay with pNPP as the substrate. Derepression of the enzyme occurred in cultures starved for > 30 d in the lake water and within 5 d in lake water microcosms supplemented with carbon and nitrogen sources where there was rarely an increase in viable count. Chloramphenicol prevented the synthesis of alkaline phosphatase suggesting that, even under starvation conditions, de novo synthesis of the enzyme occurs.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	JOURNAL OF APPLIED BACTERIOLOGY
Publisher:	BLACKWELL SCIENCE LTD
ISSN:	0021-8847
Date:	March 1996
Volume:	80
Number:	3
Number of Pages:	7
Page Range:	pp. 252-258
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19004

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