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Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme
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UNSPECIFIED (1996) Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme. MICROBIOLOGY-UK, 142 (Part 2). pp. 251-260. ISSN 1350-0872.
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Abstract
A novel methylotroph, strain M2, capable of utilizing methanesulfonic acid (MSA) as a sole source of carbon and energy was the subject of these investigations, The initial step in the biodegradative pathway of MSA in strain M2 involved an inducible NADH-specific monooxygenase enzyme (MSAMO). Partial purification of MSAMO from cell-free extracts by ion-exchange chromatography led to the loss of MSAMO activity. Activity was restored by the mixing of three distinct protein fractions designated A, B and C. The reconstituted enzyme had a narrow substrate specificity relative to crude cell-free extracts. Addition of FAD and ferrous ions to the reconstituted enzyme complex resulted in a fivefold increase in enzyme activity, suggesting the loss of FAD and ferrous ion from the multicomponent enzyme on purification. Analysis of mutants of strain M2 defective in the metabolism of C-1 compounds indicated that methanol was not an intermediate in the degradative pathway of MSA and also confirmed the involvement of a multicomponent enzyme in the degradation of MSA by methylotroph strain M2.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | MICROBIOLOGY-UK | ||||
Publisher: | SOC GENERAL MICROBIOLOGY | ||||
ISSN: | 1350-0872 | ||||
Official Date: | February 1996 | ||||
Dates: |
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Volume: | 142 | ||||
Number: | Part 2 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 251-260 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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