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Characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice
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UNSPECIFIED (1995) Characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice. VIRUS RESEARCH, 39 (2-3). pp. 221-235. ISSN 0168-1702.
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Abstract
A protein blotting technique was used to study the interaction occurring between the pneumonia virus of mice N protein and other PVM encoded proteins expressed in infected cells. Measurement of the degree of binding indicated that the N protein specifically interacted only with the full-length 39 kDa P protein in infected cells. Truncated N-related proteins were synthesised in vitro and incubated with filter-bound full-length and truncated P proteins. The data suggested that many regions of the N protein are cooperatively involved in the binding process. It was also determined that both the amino and the carboxyl-terminal regions of the PVM P protein were essential for binding to N protein.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||
| Journal or Publication Title: | VIRUS RESEARCH | ||||
| Publisher: | ELSEVIER SCIENCE BV | ||||
| ISSN: | 0168-1702 | ||||
| Official Date: | December 1995 | ||||
| Dates: |
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| Volume: | 39 | ||||
| Number: | 2-3 | ||||
| Number of Pages: | 15 | ||||
| Page Range: | pp. 221-235 | ||||
| Publication Status: | Published |
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