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Characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice

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UNSPECIFIED (1995) Characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice. VIRUS RESEARCH, 39 (2-3). pp. 221-235. ISSN 0168-1702.

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Abstract

A protein blotting technique was used to study the interaction occurring between the pneumonia virus of mice N protein and other PVM encoded proteins expressed in infected cells. Measurement of the degree of binding indicated that the N protein specifically interacted only with the full-length 39 kDa P protein in infected cells. Truncated N-related proteins were synthesised in vitro and incubated with filter-bound full-length and truncated P proteins. The data suggested that many regions of the N protein are cooperatively involved in the binding process. It was also determined that both the amino and the carboxyl-terminal regions of the PVM P protein were essential for binding to N protein.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title: VIRUS RESEARCH
Publisher: ELSEVIER SCIENCE BV
ISSN: 0168-1702
Official Date: December 1995
Dates:
DateEvent
December 1995UNSPECIFIED
Volume: 39
Number: 2-3
Number of Pages: 15
Page Range: pp. 221-235
Publication Status: Published

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