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DETECTION OF CIRCULATING TUMOR-CELLS WITH THE MAGNETIC ACTIVATED CELL SORTER
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UNSPECIFIED (1995) DETECTION OF CIRCULATING TUMOR-CELLS WITH THE MAGNETIC ACTIVATED CELL SORTER. BRITISH JOURNAL OF SURGERY, 82 (10). pp. 1333-1337. ISSN 0007-1323.
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Abstract
A technique has been developed for the detection of tumour cells in blood with the magnetic activated cell sorter (MACS). Colonic carcinoma cell lines and disaggregated primary tumours were used to establish optimal conditions of separation. A murine monoclonal antibody specific for epithelial cells was added to the suspension of leucocytes and tumour cells, followed by magnetic labelled goat antimouse antibody. The labelled tumour cells were retrieved by passing this suspension through a MACS separation column in a strong magnetic field. Tumour dells were detected at a dilution of 10 cells per ml blood. Tumour cells were identified in mesenteric blood in three of 24 patients undergoing surgery for colorectal cancer. This study supports the use of the MACS to detect circulating tumour cells.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RD Surgery | ||||
Journal or Publication Title: | BRITISH JOURNAL OF SURGERY | ||||
Publisher: | BLACKWELL SCIENCE LTD | ||||
ISSN: | 0007-1323 | ||||
Official Date: | October 1995 | ||||
Dates: |
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Volume: | 82 | ||||
Number: | 10 | ||||
Number of Pages: | 5 | ||||
Page Range: | pp. 1333-1337 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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