UNSPECIFIED (1995) HEAT-TOLERANT METHANOTROPHIC BACTERIA FROM THE HOT-WATER EFFLUENT OF A NATURAL-GAS FIELD. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 61 (10). pp. 3549-3555. ISSN 0099-2240

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Abstract

Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene duster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 mu M copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 mu M copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology
Journal or Publication Title:	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0099-2240
Date:	October 1995
Volume:	61
Number:	10
Number of Pages:	7
Page Range:	pp. 3549-3555
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19404

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