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SELECTIVE RECOVERY OF STREPTOSPORANGIUM FRAGILE FROM SOIL BY INDIRECT IMMUNOMAGNETIC CAPTURE
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UNSPECIFIED (1995) SELECTIVE RECOVERY OF STREPTOSPORANGIUM FRAGILE FROM SOIL BY INDIRECT IMMUNOMAGNETIC CAPTURE. MICROBIOLOGY-UK, 141 (Part 9). pp. 2149-2156. ISSN 1350-0872.
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Abstract
A polyclonal antibody raised to Streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of Streptosporangium, as determined by dot immunoblotting. An indirect immunomagnetic capture method was developed for the recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit M-280 Dynabeads. The effects of different soil blocking agents, antibody labelling concentrations and spore/Dynabead capture times on the recovery of S. fragile spores were investigated. Pre-blocking of antibody binding sites within the soil, with either 2% partially hydrolysed gelatin or 10% skimmed milk, was essential prior to immunomagnetic capture, Increasing the capture time from 15 to 60 min did not affect spore recovery; however, a 10-fold decline in the magnetic bead concentration did result in a significantly lower recovery of spores from soil. S, fragile was selectively enriched (1:190-fold) when present as a mixed population with Arthrobacter oxydans in sterile soil. The indirect immunomagnetic capture method was used to selectively recover S. fragile spores seeded into non-sterile soil, although some background binding of non-target bacteria was noted. The target was successfully recovered from a sterile soil microcosm after 14 d incubation and the capture rate was increased by the inclusion of an initial soil dispersion and biomass concentration procedure, using the ion-exchange resin Chelex 100.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | MICROBIOLOGY-UK | ||||
Publisher: | SOC GENERAL MICROBIOLOGY | ||||
ISSN: | 1350-0872 | ||||
Official Date: | September 1995 | ||||
Dates: |
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Volume: | 141 | ||||
Number: | Part 9 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 2149-2156 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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