SELECTIVE RECOVERY OF STREPTOSPORANGIUM FRAGILE FROM SOIL BY INDIRECT IMMUNOMAGNETIC CAPTURE
UNSPECIFIED (1995) SELECTIVE RECOVERY OF STREPTOSPORANGIUM FRAGILE FROM SOIL BY INDIRECT IMMUNOMAGNETIC CAPTURE. MICROBIOLOGY-UK, 141 (Part 9). pp. 2149-2156. ISSN 1350-0872Full text not available from this repository.
A polyclonal antibody raised to Streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of Streptosporangium, as determined by dot immunoblotting. An indirect immunomagnetic capture method was developed for the recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit M-280 Dynabeads. The effects of different soil blocking agents, antibody labelling concentrations and spore/Dynabead capture times on the recovery of S. fragile spores were investigated. Pre-blocking of antibody binding sites within the soil, with either 2% partially hydrolysed gelatin or 10% skimmed milk, was essential prior to immunomagnetic capture, Increasing the capture time from 15 to 60 min did not affect spore recovery; however, a 10-fold decline in the magnetic bead concentration did result in a significantly lower recovery of spores from soil. S, fragile was selectively enriched (1:190-fold) when present as a mixed population with Arthrobacter oxydans in sterile soil. The indirect immunomagnetic capture method was used to selectively recover S. fragile spores seeded into non-sterile soil, although some background binding of non-target bacteria was noted. The target was successfully recovered from a sterile soil microcosm after 14 d incubation and the capture rate was increased by the inclusion of an initial soil dispersion and biomass concentration procedure, using the ion-exchange resin Chelex 100.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||MICROBIOLOGY-UK|
|Publisher:||SOC GENERAL MICROBIOLOGY|
|Number of Pages:||8|
|Page Range:||pp. 2149-2156|
Actions (login required)