UNSPECIFIED. (1995) LYMPHOCYTES INFILTRATING COLORECTAL-CANCER HAVE LOW PROLIFERATIVE CAPACITY BUT CAN SECRETE NORMAL LEVELS OF INTERFERON-GAMMA. CANCER IMMUNOLOGY IMMUNOTHERAPY, 41 (1). pp. 61-67. ISSN 0340-7004

Abstract

Significant numbers of infiltrating mononuclear cells are commonly observed in solid tumours although their role in restricting tumour growth is not clear. Tumour-infiltrating lymphocytes (TIL) from 38 patients with colorectal cancer in parallel with peripheral blood lymphocytes (PBL), were assayed to determine their ability to proliferate in response to concanavalin A (ConA) interleukin-2 (IL-1). ConA + IL-2 phorbol 12-myristate 13-acetate (PMA) - ionomycin (IOM) and staphylococcal enterotoxin B (SEB). These reagents were selected to give a ranee of independent of the T cell receptor. Proliferation of TIL was significantly lower than that of PBL in all cultures: ConA + IL-2 (P < 0.001) PMA + IOM (P < 0.001) SEB (P = 0.002). In addition to the low proliferative capacity of TIL production of cytokines by TIL may also play a role in control oi tumour growth. We have assayed IFN gamma production in the supernatants from 16 paired TIL and PBL cultures and tumour necrosis factor alpha((TNF-alpha) in 6 paired cultures. TNF alpha concentrations were significantly lower in TIL cultures than in PBL cultures stimulated with ConA (P < 0.05), but no different in control or IL-2 stimulated cultures. IFN gamma levels did not significantly differ between PBL and TIL cultures, indicating that despite the restricted proliferative capacity of TIL, these cells remain capable of secreting significant amounts of IFN gamma.

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