UNSPECIFIED (1995) ACTIVATION BY INTERFERON-GAMMA OF EXPRESSION OF ICAM-1 AND MHC CLASS-II ANTIGENS IN TUMOR-CELLS FROM COLORECTAL CARCINOMAS. JOURNAL OF CLINICAL PATHOLOGY-CLINICAL MOLECULAR PATHOLOGY EDITION, 48 (1). M40-M45. ISSN 1355-2910

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Abstract

Aims-To determine whether lack of MHC class IP antigen and intercellular adhesion molecule-1 (ICAM-1) expression in some tumours is due to the inability of the tumour cells to respond to the cytokine interferon-gamma (IFN-gamma), an important activator of these surface molecules. Methods-Cells from 40 colorectal tumours which did not constitutively express class II MHC antigens or ICAM-1 were kept in short term culture after disaggregation for a few days to two weeks without significant loss of viability. These were treated with IFN-gamma. Expression of class II MHC antigens and ICAM-1 was determined using immunohistological techniques. Results-There was clear induction in vitro of both MHC class II antigens and ICAM-1 in cells from eight of the tumours, with between 50 and 80% of the tumour cells in the cultures staining positively. The staining was apparent within 24 hours, appeared maximal at about three days, and declined thereafter. There were no obvious differences in cell. morphology or viability between the cultures which were inducible and those which were not, nor were there obvious differences between the tumours from which they were derived. Conclusions-Expression of MHC class II antigens and ICAM-1 may be induced by IFN-gamma in a small proportion of colorectal tumours which do not constitutively express these antigens, showing that only a minority of tumours are capable of responding to this cytokine.

Item Type:	Journal Article
Subjects:	R Medicine > RB Pathology
Journal or Publication Title:	JOURNAL OF CLINICAL PATHOLOGY-CLINICAL MOLECULAR PATHOLOGY EDITION
Publisher:	BRITISH MED JOURNAL PUBL GROUP
ISSN:	1355-2910
Date:	February 1995
Volume:	48
Number:	1
Number of Pages:	6
Page Range:	M40-M45
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19564

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