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A SECY HOMOLOG IN ARABIDOPSIS-THALIANA - SEQUENCE OF A FULL-LENGTH CDNA CLONE AND IMPORT OF THE PRECURSOR PROTEIN INTO CHLOROPLASTS
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UNSPECIFIED (1995) A SECY HOMOLOG IN ARABIDOPSIS-THALIANA - SEQUENCE OF A FULL-LENGTH CDNA CLONE AND IMPORT OF THE PRECURSOR PROTEIN INTO CHLOROPLASTS. JOURNAL OF BIOLOGICAL CHEMISTRY, 270 (30). pp. 17664-17667.
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Abstract
Proteins are translocated across the thylakoid membrane by two distinct pathways in higher plant chloroplasts, one of which is related to prokaryotic Sec-dependent translocation mechanisms. SecY is an essential, hydrophobic component of the membrane-bound translocase complex in bacteria, and we report here the nucleotide sequence of a full-length cDNA encoding a homolog of SecY from Arabidopsis thaliana. The predicted protein of 551 residues includes an amino-terminal extension of approximately 120 residues when compared with other SecY proteins. The deduced sequence of the mature protein, cpSecY, is 41% identical with SecY from Synechococcus and 33% identical with the Escherichia coli protein. The extension serves to target the protein into chloroplasts; transcription-translation of the cDNA yields a 58-kDa precursor protein which is imported into pea chloroplasts, processed to a product of 46 kDa, and targeted into the thylakoid membrane.
Item Type: | Journal Item | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | JOURNAL OF BIOLOGICAL CHEMISTRY | ||||
Publisher: | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | ||||
ISSN: | 0021-9258 | ||||
Official Date: | 28 July 1995 | ||||
Dates: |
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Volume: | 270 | ||||
Number: | 30 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 17664-17667 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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