UNSPECIFIED (1995) INTERACTION BETWEEN PRIMARY AND SECONDARY METABOLISM IN STREPTOMYCES-COELICOLOR A3(2) - ROLE OF PYRROLINE-5-CARBOXYLATE DEHYDROGENASE. MICROBIOLOGY-UK, 141 (Part 7). pp. 1739-1744. ISSN 1350-0872

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Abstract

The activity of the proline catabolic enzyme pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) was induced up to threehundredfold by the addition of three hundred proline to the growth medium of the Gram-positive bacterium Streptomyces coelicolor A3(2). Rifampicin, an inhibitor of RNA polymerase activity, abolished induction, implying that regulation was at the level of activation of gene transcription. The enzyme was purified and SDS-PAGE of the highly purified enzyme preparation revealed a single subunit with M(r) 68 000. A single band of protein, which also stained for enzyme activity, was observed after native gel electrophoresis. The M(r) of the enzyme was estimated to be approximately 265 000 by native gel electrophoresis and approximately 305 000 by gel filtration, which indicated that the enzyme had a tetrameric quaternary structure. The apparent K-m for pyrroline-5-carboxylate was 109 +/- 7.3 mu M, whilst that for NAD(+) was 43.3 +/- 2.5 mu M. Product inhibition by NADH (apparent K-i 0.6 mM) was observed. The observed V-max was 22.0 +/- 1 mol min(-1) (mg protein)(-1). Neither 1 nor 5 mM proline had any effect on enzyme activity, whilst glutamate was a very weak inhibitor.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	MICROBIOLOGY-UK
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	1350-0872
Date:	July 1995
Volume:	141
Number:	Part 7
Number of Pages:	6
Page Range:	pp. 1739-1744
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/19581

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